It now seems certain that the amino acid sequence of any protein is determined by the sequence of bases in some region of a particular nucleic acid molecule.
Jim and I hit it off immediately, partly because our interests were astonishingly similar and partly, I suspect, because a certain youthful arrogance, a ruthlessness, an impatience with sloppy thinking can naturally to both of us.
Do codons overlap? In other words, as we read along the genetic message do we find a base which is a member of two or more codons? It now seems fairly certain that codons do not overlap.
A final proof of our ideas can only be obtained by detailed studies on the alterations produced in the amino acid sequence of a protein by mutations of the type discussed here.
It would appear that the number of nonsense triplets is rather low, since we only occasionally come across them. However this conclusion is less secure than our other deductions about the general nature of the genetic code.
Exact knowledge is the enemy of vitalism.
It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.
Biologists must constantly keep in mind that what they see was not designed, but rather evolved.
Evolution is cleverer than you are.
Christianity may be OK between consenting adults in private but should not be taught to young children.
It is one of the more striking generalizations of biochemistry – which surprisingly is hardly ever mentioned in the biochemical textbooks – that the twenty amino acids and the four bases, are, with minor reservations, the same throughout Nature.
Almost all aspects of life are engineered at the molecular level, and without understanding molecules we can only have a very sketchy understanding of life itself.
Chance is the only source of true novelty.
It is notoriously difficult to define the word living.
This seems highly likely, especially as it has been shown that in several systems mutations affecting the same amino acid are extremely near together on the genetic map.
Trying to determine the structure of a protein by UV spectroscopy was like trying to determine the structure of a piano by listening to the sound it made while being dropped down a flight of stairs.
If, for example, all the codons are triplets, then in addition to the correct reading of the message, there are two incorrect readings which we shall obtain if we do not start the grouping into sets of three at the right place.
Again the message to experimentalists is: Be sensible but don’t be impressed too much by negative arguments. If at all possible, try it and see what turns up. Theorists almost always dislike this sort of approach.
If poly A is added to poly U, to form a double or triple helix, the combination is inactive.
Unfortunately it makes the unambiguous determination of triplets by these methods much more difficult than would be the case if there were only one triplet for each amino acid.
Anybody who believes that the earth is less than 10,000 years old needs psychiatric help.